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It becomes active when either the Concentration or Time mode is selected. List of wavelength options This column lists all the available wavelengths. You may wish to select a different wavelength. By default, the wavelength with the maximum measured value will be selected. Graph The graph displays a full-spectrum analysis of the sample in the cuvette holder. Concentration is selected, a wavelength or wavelengths will need to be chosen. If the measurement (Absorbance in this example) vs. There are three regions in this box: Collection Mode The three options for data collection are offered. Click the Configure Spectrophotometer Data Collection button. To store the spectrum data, choose Store Latest Run from the Experiment menu. Place the sample in the Spectrophotometer and click. #Logger pro absorbance fullFill a cuvette about ¾ full of a sample of the solution to be tested. concentration for Beer s law experiments, and absorbance (or %T) vs. wavelength, which produces a spectrum, absorbance (or %T) vs. Collect Data with a Computer There are three general types of data collection that measure absorbance or transmittance absorbance (or %T) vs. Follow the instructions in the dialog box to complete the calibration, and then click. Align the cuvette so the clear side of the cuvette is facing the light source. After the Spectrophotometer has warmed up, place the blank cuvette in the Spectrophotometer. Fill a cuvette about ¾ full with distilled water (or the solvent being used in the experiment) to serve as the blank. Note: For best results, allow the Spectrophotometer to warm up for a minimum of five minutes. To calibrate the SpectroVis Plus, choose Calibrate Spectrophotometer from the Experiment menu. Calibrate (Not Required if Measuring Intensity or Fluorescence) 1. Select the unit or data type you wish to measure. Choose Change Units Spectrophotometer from the Experiment menu. If you want to measure %T, fluorescence (excited at 405 nm or 500 nm), or intensity, do the following: 1. If you want to measure the absorbance of a solution, proceed directly to the Calibrate section below. Select the Type of Data (or Units) You Want to Measure The default data type is absorbance. #Logger pro absorbance softwareStart Logger Pro software on your computer. #Logger pro absorbance driversThe device drivers were installed when you installed Logger Pro. Note: The first time you connect a Spectrophotometer, your computer may ask you a few questions. Connect the Spectrophotometer to a powered USB port on the computer or a powered USB hub. #Logger pro absorbance plusEnsure Logger Pro software (version or newer) is installed on your computer before using the SpectroVis Plus Spectrophotometer. Use the Spectrophotometer with a Computer (For LabQuest instructions, see page 6) Getting Started 1. Do not use this instrument for clinical or diagnostic procedures. Contact Vernier Technical Support for troubleshooting and technical assistance. Do not use this instrument if it is damaged in any way. This instrument can be damaged if it is dropped. Contact Vernier for all repairs and service including lamp replacement. Do not attempt to open or modify this device. No user-serviceable parts are in this device. ![]() Doing so will create an unsafe operating condition and will void the product warranty. Do not remove or modify any of the installed safety components of this device. Continue to use proper safety precautions. Safety When this instrument is in data-collection mode labeled Intensity, the light source will be blocked or turned off. ![]() Our products are not designed nor are they recommended for any industrial, medical, or commercial process such as life support, patient diagnosis, control of a manufacturing process, or industrial testing of any kind. NOTE: Vernier products are designed for educational use. #Logger pro absorbance updateVisit the downloads section of to update your software. LabQuest App 1.4, or newer, is required if you are using the original LabQuest. LabQuest App version 2.0, or newer, is required if you are using LabQuest 2. What is Included with the SpectroVis Plus Spectrophotometer? One SpectroVis Plus unit One package of 15 plastic cuvettes and lids USB cable Software Requirements Logger Pro 3 (version or newer) software is required if you are using a computer. 1 Vernier SpectroVis Plus Spectrophotometer (Order Code: SVIS-PL) SpectroVis Plus is a portable, visible to near-ir spectrophotometer and fluorometer. ![]()
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